ha tagged rictor (Addgene inc)
Structured Review
Ha Tagged Rictor, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/myc+rictor+plasmid/Rictor-Myc+(Plasmid+%23157931)/pm41787128-322-6-10
Average 92 stars, based on 6 article reviews
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Plasmid Preparation:Article Title: Phosphoproteomic Analysis Defines BABAM1 as mTORC2 Downstream Effector Promoting DNA Damage Response in Glioblastoma Cells. Article Snippet: Glioblastoma (GBM) is a devastating primary brain cancer with a poor prognosis.. GBM is associated with an abnormal mechanistic target of rapamycin (mTOR) signaling pathway, consisting of two distinct kinase complexes: mTORC1 and mTORC2.. The complexes play critical roles in cell proliferation, survival, migration, metabolism, and DNA damage response. Article Title: Genetic immunodeficiency and autoimmune disease reveal distinct roles of Hem1 in the WAVE2 and mTORC2 complexes Article Snippet: For Hem1 overexpression experiments, 2×10 6 HEK293T cells were transiently transfected with 20 μg Hem1-3xFLAG plasmid DNA using Lipofectamine 3000 reagent (Thermo Fisher) and cultured for 72 hours before harvest. .. For Rictor overexpression experiments, 2×10 6 HEK293T cells stably expressing FLAG-tagged Hem1 were transiently transfected with 2 μg Over Expression:Article Title: Genetic immunodeficiency and autoimmune disease reveal distinct roles of Hem1 in the WAVE2 and mTORC2 complexes Article Snippet: For Hem1 overexpression experiments, 2×10 6 HEK293T cells were transiently transfected with 20 μg Hem1-3xFLAG plasmid DNA using Lipofectamine 3000 reagent (Thermo Fisher) and cultured for 72 hours before harvest. .. For Rictor overexpression experiments, 2×10 6 HEK293T cells stably expressing FLAG-tagged Hem1 were transiently transfected with 2 μg Stable Transfection:Article Title: Genetic immunodeficiency and autoimmune disease reveal distinct roles of Hem1 in the WAVE2 and mTORC2 complexes Article Snippet: For Hem1 overexpression experiments, 2×10 6 HEK293T cells were transiently transfected with 20 μg Hem1-3xFLAG plasmid DNA using Lipofectamine 3000 reagent (Thermo Fisher) and cultured for 72 hours before harvest. .. For Rictor overexpression experiments, 2×10 6 HEK293T cells stably expressing FLAG-tagged Hem1 were transiently transfected with 2 μg Expressing:Article Title: Genetic immunodeficiency and autoimmune disease reveal distinct roles of Hem1 in the WAVE2 and mTORC2 complexes Article Snippet: For Hem1 overexpression experiments, 2×10 6 HEK293T cells were transiently transfected with 20 μg Hem1-3xFLAG plasmid DNA using Lipofectamine 3000 reagent (Thermo Fisher) and cultured for 72 hours before harvest. .. For Rictor overexpression experiments, 2×10 6 HEK293T cells stably expressing FLAG-tagged Hem1 were transiently transfected with 2 μg Transfection:Article Title: Genetic immunodeficiency and autoimmune disease reveal distinct roles of Hem1 in the WAVE2 and mTORC2 complexes Article Snippet: For Hem1 overexpression experiments, 2×10 6 HEK293T cells were transiently transfected with 20 μg Hem1-3xFLAG plasmid DNA using Lipofectamine 3000 reagent (Thermo Fisher) and cultured for 72 hours before harvest. .. For Rictor overexpression experiments, 2×10 6 HEK293T cells stably expressing FLAG-tagged Hem1 were transiently transfected with 2 μg |
